Trim22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81795

Novus Biologicals, part of Bio-Techne
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NBP1-81795
NBP1-81795-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Trim22 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NEVVKECQEKLQVALQRLIKEDQEAEKLEDDIRQERTAWKNYIQIERQKILKGFNEMRVILDNEEQRELQKLEEGEVNVLDNLAAATDQLVQQRQDASTLISDLQRRLRGSSVEMLQDVIDVMKRSESWTLKKPKSVSKKLKSVFRV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23448979)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Trim22 Antibody

Western Blot: Trim22 Antibody [NBP1-81795]

Western Blot: Trim22 Antibody [NBP1-81795]

Western Blot: Trim22 Antibody [NBP1-81795] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-TRIM22 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.
Immunocytochemistry/ Immunofluorescence: Trim22 Antibody [NBP1-81795]

Immunocytochemistry/ Immunofluorescence: Trim22 Antibody [NBP1-81795]

Immunocytochemistry/Immunofluorescence: Trim22 Antibody [NBP1-81795] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm, nuclear bodies & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Trim22 Antibody [NBP1-81795]

Immunohistochemistry-Paraffin: Trim22 Antibody [NBP1-81795]

Immunohistochemistry-Paraffin: Trim22 Antibody [NBP1-81795] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
View 4 more images

Applications for Trim22 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Trim22

Trim22 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. [provided by RefSeq]

Alternate Names

50 kDa-stimulated trans-acting factor, E3 ubiquitin-protein ligase TRIM22, EC 6.3.2, EC 6.3.2.-, GPSTAF50, RING finger protein 94, RNF94stimulated trans-acting factor (50 kDa), Staf-50, STAF50staf-50, tripartite binding motif 22, tripartite motif containing 22, tripartite motif protein TRIM22, tripartite motif-containing 22, Tripartite motif-containing protein 22

Gene Symbol

TRIM22

Additional Trim22 Products

Product Documents for Trim22 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Trim22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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