TRIM25 Antibody (JE57-43)

Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053]

Western Blot: TRIM25 Antibody (JE57-43) [NBP3-33053] - Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: A549 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 71 kDa
Observed band size: 71 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33053) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (JE57-43) [NBP3-33053]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody (JE57-43) [NBP3-33053] - Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody (NBP3-33053) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.