Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TRIM25 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25. The immunogen is located within amino acids 520 - 570 of TRIM25. Amino Acid Squence: CYGSMNRQGPESRLGRNSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM25 Antibody - BSA Free

Western Blot: TRIM25 AntibodyBSA Free [NBP1-76825]

Western Blot: TRIM25 AntibodyBSA Free [NBP1-76825]

Western Blot: TRIM25 Antibody [NBP1-76825] - HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with TRIM25 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825]

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody - BSA Free [NBP1-76825]

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-76825] - HeLa cells with TRIM25 antibody at 20 ug/ml.
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Applications for TRIM25 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM25

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Gene Symbol

TRIM25

UniProt

NP_005073 (Human)

Additional TRIM25 Products

Product Documents for TRIM25 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM25 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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