TRIM25 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84891
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRIM25 Antibody
Western Blot: TRIM25 Antibody [NBP1-84891]
Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissueImmunocytochemistry/ Immunofluorescence: TRIM25 Antibody [NBP1-84891]
Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-84891] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891]
Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.Applications for TRIM25 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRIM25
Long Name
Tripartite Motif-containing Protein 25
Alternate Names
EFP, RNF147, ZNF147
Gene Symbol
TRIM25
Additional TRIM25 Products
Product Documents for TRIM25 Antibody
Product Specific Notices for TRIM25 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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