TRIM25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84891

Novus Biologicals, part of Bio-Techne
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NBP1-84891
NBP1-84891-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM25 Antibodies »

Product Summary for TRIM25 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM25 Antibody

Western Blot: TRIM25 Antibody [NBP1-84891]

Western Blot: TRIM25 Antibody [NBP1-84891]

Western Blot: TRIM25 Antibody [NBP1-84891] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody [NBP1-84891]

Immunocytochemistry/ Immunofluorescence: TRIM25 Antibody [NBP1-84891]

Immunocytochemistry/Immunofluorescence: TRIM25 Antibody [NBP1-84891] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891]

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891]

Immunohistochemistry-Paraffin: TRIM25 Antibody [NBP1-84891] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for TRIM25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM25

TRIM25 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Long Name

Tripartite Motif-containing Protein 25

Alternate Names

EFP, RNF147, ZNF147

Gene Symbol

TRIM25

Additional TRIM25 Products

Product Documents for TRIM25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRIM25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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