TRIM3/BERP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2494
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence AKREDSPGPEVQP-C corresponding to N-Terminus according to NP_150594.2.
Specificity
Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for TRIM3/BERP Antibody
![Western Blot: TRIM3/BERP Antibody [NB100-2494]](https://resources.bio-techne.com/images/products/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0003.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494]")
Western Blot: TRIM3/BERP Antibody [NB100-2494]
Western Blot: TRIM3/BERP Antibody [NB100-2494] - Staining of Human Cerebellum (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Western Blot: TRIM3/BERP Antibody [NB100-2494]](https://resources.bio-techne.com/images/products/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0001.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494]")
Western Blot: TRIM3/BERP Antibody [NB100-2494]
Western Blot: TRIM3/BERP Antibody [NB100-2494] - HEK293 overexpressing TRIM3 and probed with (mock transfection in first lane).![Western Blot: TRIM3/BERP Antibody [NB100-2494]](https://resources.bio-techne.com/images/products/TRIM3-BERP-Antibody-Western-Blot-NB100-2494-img0002.jpg "Western Blot: TRIM3/BERP Antibody [NB100-2494]")
Western Blot: TRIM3/BERP Antibody [NB100-2494]
Western Blot: TRIM3/BERP Antibody [NB100-2494] - Western blot analysis of TRIM3/BERP in Mouse Brain lysate (RIPA buffer, 30ug total protein per lane) using NB100-2494 at 0.05ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Applications for TRIM3/BERP Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
0.1 - 1 ug/ml
Application Notes
Please note the HEK cell experiements were performed using a previous batch (different goat). WB: Approx. 85 kDa band observed in mouse brain and human brain (cerebellum) lysate (predicted MW of approx. 80.8 kDa band according to NP_006449.2).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TRIM3/BERP
Alternate Names
BERPRING finger protein 97, Brain-expressed RING finger protein, HAC1, RING finger protein 22, RNF22brain expressed ring finger, RNF97FLJ16135, tripartite motif containing 3, tripartite motif protein TRIM3, tripartite motif-containing 3, tripartite motif-containing protein 3
Gene Symbol
TRIM3
UniProt
Additional TRIM3/BERP Products
Product Documents for TRIM3/BERP Antibody
Product Specific Notices for TRIM3/BERP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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