Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRIM3/BERP Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human tripartite motif-containing 3 (brain expressed ring finger protein) using the numbering given in entry NP_006449.2 (GeneID 10612).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM3/BERP Antibody

Immunohistochemistry-Paraffin: TRIM3/BERP Antibody [NB100-60400]

Immunohistochemistry-Paraffin: TRIM3/BERP Antibody [NB100-60400]

Immunohistochemistry-Paraffin: TRIM3/BERP Antibody [NB100-60400] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- TRIM3/BERP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: TRIM3/BERP Antibody [NB100-60400]

Immunoprecipitation: TRIM3/BERP Antibody [NB100-60400]

Immunoprecipitation: TRIM3/BERP Antibody [NB100-60400] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-60400 used for IP at 3 mcg/mg lysate. TRIM3/BERP was also immunoprecipitated by rabbit anti-TRIM3/BERP antibodies NB100-60401 and NB100-60402, which recognize downstream epitopes.

Applications for TRIM3/BERP Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRIM3/BERP

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.

Alternate Names

BERPRING finger protein 97, Brain-expressed RING finger protein, HAC1, RING finger protein 22, RNF22brain expressed ring finger, RNF97FLJ16135, tripartite motif containing 3, tripartite motif protein TRIM3, tripartite motif-containing 3, tripartite motif-containing protein 3

Entrez Gene IDs

10612 (Human)

Gene Symbol

TRIM3

UniProt

NP_006449.2 (Human)

Additional TRIM3/BERP Products

Product Documents for TRIM3/BERP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRIM3/BERP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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