TRIM32 Antibody (2E5)
Novus Biologicals, part of Bio-Techne | Catalog # H00022954-M09
![ELISA: TRIM32 Antibody (2E5) [H00022954-M09]](https://resources.bio-techne.com/images/products/TRIM32-Antibody-2E5-Sandwich-ELISA-H00022954-M09-img0005.jpg "ELISA: TRIM32 Antibody (2E5) [H00022954-M09]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockdown Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2E5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TRIM32 (NP_036342, 105 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRK
Specificity
TRIM32 (2E5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for TRIM32 Antibody (2E5)
![Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]](https://resources.bio-techne.com/images/products/TRIM32-Antibody-2E5-Western-Blot-H00022954-M09-img0006.jpg "Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]")
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09]](https://resources.bio-techne.com/images/products/TRIM32-Antibody-2E5-Immunocytochemistry-Immunofluorescence-H00022954-M09-img0004.jpg "Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09]")
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09]
Immunocytochemistry/Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml![Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]](https://resources.bio-techne.com/images/products/TRIM32-Antibody-2E5-Western-Blot-H00022954-M09-img0002.jpg "Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]")
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09]
Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH (36.1 kDa) used as loading control.Applications for TRIM32 Antibody (2E5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TRIM32
Long Name
Tripartite Motif-containing Protein 32
Alternate Names
BBS11, HT2A, LGMD2H, TATIP
Entrez Gene IDs
22954 (Human)
Gene Symbol
TRIM32
OMIM
254110 (Human)
UniProt
Additional TRIM32 Products
Product Documents for TRIM32 Antibody (2E5)
Product Specific Notices for TRIM32 Antibody (2E5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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