TRIM32 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33737

Novus Biologicals, part of Bio-Techne
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NBP1-33737
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Feline (98%), Rat (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM32 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM32. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Cat (98%), Bovine (88%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM32 Antibody

Western Blot: TRIM32 Antibody [NBP1-33737]

Western Blot: TRIM32 Antibody [NBP1-33737]

Western Blot: TRIM32 Antibody [NBP1-33737] - A. 30 ug 293T whole cell lysate/extract 7.5 % SDS-PAGETRIM32 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP1-33737]

Immunocytochemistry/ Immunofluorescence: TRIM32 Antibody [NBP1-33737]

Immunocytochemistry/Immunofluorescence: TRIM32 Antibody [NBP1-33737] - A375 cells were fixed in ice-cold MeOH for 5 min. Green: TRIM32 protein stained by TRIM32 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP1-33737]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP1-33737]

Immunohistochemistry-Paraffin: TRIM32 Antibody [NBP1-33737] - Mouse brain diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
View 1 more images

Applications for TRIM32 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM32

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.

Long Name

Tripartite Motif-containing Protein 32

Alternate Names

BBS11, HT2A, LGMD2H, TATIP

Gene Symbol

TRIM32

Additional TRIM32 Products

Product Documents for TRIM32 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM32 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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