TRIM5 alpha Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76600

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TRIM5 alpha Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha. The immunogen is located within the last 50 amino acids of TRIM5 alpha. Amino Acid Squence: SFSKPVFPYLNPRK

Specificity

Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM5 alpha Antibody - BSA Free

Western Blot: TRIM5 alpha AntibodyBSA Free [NBP1-76600]

Western Blot: TRIM5 alpha AntibodyBSA Free [NBP1-76600]

Western Blot: TRIM5 alpha Antibody [NBP1-76600] - Analysis of TRIM5 a expression in human stomach (A), thymus (B), and uterus (C) cell lysate with anti-TRIM5 a using this antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TRIM5 alpha Antibody - BSA Free [NBP1-76600]

Immunocytochemistry/ Immunofluorescence: TRIM5 alpha Antibody - BSA Free [NBP1-76600]

Immunocytochemistry/Immunofluorescence: TRIM5 alpha Antibody [NBP1-76600] - Human breast tissue with TRIM5 alpha antibody at 10 ug/ml.

Applications for TRIM5 alpha Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM5 alpha

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.

Alternate Names

EC 6.3.2, EC 6.3.2.-, RING finger protein 88, RNF88tripartite motif protein TRIM, TRIM5alpha, tripartite motif containing 5, tripartite motif protein TRIM5, tripartite motif-containing 5, tripartite motif-containing protein 5

Gene Symbol

TRIM5

UniProt

NP_149023 (Human)

Additional TRIM5 alpha Products

Product Documents for TRIM5 alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRIM5 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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