TRIM72 Antibody (A1D4-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33056
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A1D4-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein with Human TRIM72 aa 50-150 / 477. (Uniprot: Q6ZMU5)
Localization
Sarcolemma, cytoplasmic vesicle membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TRIM72 Antibody (A1D4-R)
Western Blot: TRIM72 Antibody (A1D4-R) [NBP3-33056]
Western Blot: TRIM72 Antibody (A1D4-R) [NBP3-33056] - Western blot analysis of TRIM72 on different lysates with Mouse anti-TRIM72 antibody (NBP3-33056) at 1/1,000 dilution.Lane 1: HCT 116 cell lysate (20 ug/Lane)
Lane 2: Mouse skeletal muscle tissue lysate (40 ug/Lane)
Lane 3: Mouse heart tissue lysate (40 ug/Lane)
Lane 4: Rat heart tissue lysate (40 ug/Lane)
Lane 5: Rat skeletal muscle tissue lysate (40 ug/Lane)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33056) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Applications for TRIM72 Antibody (A1D4-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRIM72
Alternate Names
MG53Mg53, Mitsugumin-53, tripartite motif containing 72, tripartite motif-containing 72, tripartite motif-containing protein 72
Gene Symbol
TRIM72
Additional TRIM72 Products
Product Documents for TRIM72 Antibody (A1D4-R)
Product Specific Notices for TRIM72 Antibody (A1D4-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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