Triosephosphate isomerase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56753

Novus Biologicals, part of Bio-Techne
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NBP2-56753
NBP2-56753-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Triosephosphate isomerase Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: GEEAEFHFAALYISGQWPRLRADTDLQRLGSSAMAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NBP2-56753]

Western Blot: Triosephosphate isomerase Antibody [NBP2-56753]

Western Blot: Triosephosphate isomerase Antibody [NBP2-56753] - Analysis using Anti-TPI1 antibody NBP2-56753 (A) shows similar pattern to independent antibody NBP2-58150 (B).
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP2-56753]

Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP2-56753]

Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody [NBP2-56753] - Staining of human cell line MCF7 shows localization to nucleus & vesicles.
Immunohistochemistry: Triosephosphate isomerase Antibody [NBP2-56753]

Immunohistochemistry: Triosephosphate isomerase Antibody [NBP2-56753]

Immunohistochemistry: Triosephosphate isomerase Antibody [NBP2-56753] - Immunohistochemical staining of human parathyroid gland shows moderate nuclear positivity in glandular cells.

Applications for Triosephosphate isomerase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Gene Symbol

TPI1

Additional Triosephosphate isomerase Products

Product Documents for Triosephosphate isomerase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Triosephosphate isomerase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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