TrkB [p Tyr817] Antibody (SC0556)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67578
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC0556
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of Human TrkB aa 789-822 / 822. (SwissProt: Q16620 Human; SwissProt: P15209 Mouse; SwissProt: Q63604 Rat)
Modification
p Tyr817
Localization
Cell membrane, Endosome membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TrkB [p Tyr817] Antibody (SC0556)
Western Blot: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Western blot analysis of TrkB on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb
Immunocytochemistry/Immunofluorescence: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Staining phospho -TrkB(Y817) in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TrkB [p Tyr817] Antibody (SC0556) [NBP2-67578] - Analysis of paraffin-embedded mouse pancreas tissue using anti- phospho -TrkB(Y817) antibody. Counter stained with hematoxylin.
Applications for TrkB [p Tyr817] Antibody (SC0556)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TrkB
Long Name
Neurotrophic Tyrosine Kinase Receptor B
Alternate Names
NTRK2
Gene Symbol
NTRK2
Additional TrkB Products
Product Documents for TrkB [p Tyr817] Antibody (SC0556)
Product Specific Notices for TrkB [p Tyr817] Antibody (SC0556)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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