TRMT12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83633

Novus Biologicals, part of Bio-Techne
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NBP1-83633
NBP1-83633-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRMT12 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WFTQRYREYLQRQKLFDTQHRVEKMPDGSVALPVLGETLPEQHLQELRNRVAPGSPCMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRMT12 Antibody

Western Blot: TRMT12 Antibody [NBP1-83633]

Western Blot: TRMT12 Antibody [NBP1-83633]

Western Blot: TRMT12 Antibody [NBP1-83633] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: TRMT12 Antibody [NBP1-83633]

Immunocytochemistry/ Immunofluorescence: TRMT12 Antibody [NBP1-83633]

Immunocytochemistry/Immunofluorescence: TRMT12 Antibody [NBP1-83633] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: TRMT12 Antibody [NBP1-83633]

Immunohistochemistry-Paraffin: TRMT12 Antibody [NBP1-83633]

Immunohistochemistry-Paraffin: TRMT12 Antibody [NBP1-83633] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Applications for TRMT12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRMT12

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM]

Alternate Names

Alpha-amino-alpha-carboxypropyl transferase TYW2, EC 2.1.1, EC 2.1.1.32, EC 2.5.1.-, FLJ20772, homolog of yeast tRNA methyltransferase, TRM12, tRNA methyltranferase 12 homolog, tRNA methyltranferase 12 homolog (S. cerevisiae), tRNA methyltransferase 12 homolog (S. cerevisiae), tRNA-yW synthesizing protein 2, tRNA-yW-synthesizing protein 2, TYW2tRNA wybutosine-synthesizing protein 2 homolog

Gene Symbol

TRMT12

Additional TRMT12 Products

Product Documents for TRMT12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRMT12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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