TRMT5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82909
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TRMT5 Antibody
Western Blot: TRMT5 Antibody [NBP1-82909]
Western Blot: TRMT5 Antibody [NBP1-82909] - Analysis in control (vector only transfected HEK293T lysate) and TRMT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: TRMT5 Antibody [NBP1-82909]
Immunocytochemistry/Immunofluorescence: TRMT5 Antibody [NBP1-82909] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909]
Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human fallopian tube shows strong nuclear postivity in glandular cells.Applications for TRMT5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICCIF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRMT5
Alternate Names
KIAA1393EC 2.1.1.31, M1G-methyltransferase, TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae), TRM5MGC111453, tRNA (guanine-N(1)-)-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5, tRNA-N1G37 methyltransferase
Gene Symbol
TRMT5
Additional TRMT5 Products
Product Documents for TRMT5 Antibody
Product Specific Notices for TRMT5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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