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TRMT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82909

Novus Biologicals, part of Bio-Techne
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NBP1-82909
NBP1-82909-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRMT5 Antibody

Western Blot: TRMT5 Antibody [NBP1-82909]

Western Blot: TRMT5 Antibody [NBP1-82909]

Western Blot: TRMT5 Antibody [NBP1-82909] - Analysis in control (vector only transfected HEK293T lysate) and TRMT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TRMT5 Antibody [NBP1-82909]

Immunocytochemistry/ Immunofluorescence: TRMT5 Antibody [NBP1-82909]

Immunocytochemistry/Immunofluorescence: TRMT5 Antibody [NBP1-82909] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909]

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909]

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human fallopian tube shows strong nuclear postivity in glandular cells.

Applications for TRMT5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICCIF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRMT5

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 (PubMed 15248782)).(supplied by OMIM)

Alternate Names

KIAA1393EC 2.1.1.31, M1G-methyltransferase, TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae), TRM5MGC111453, tRNA (guanine-N(1)-)-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5, tRNA-N1G37 methyltransferase

Gene Symbol

TRMT5

Additional TRMT5 Products

Product Documents for TRMT5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRMT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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