tropomyosin-2 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95197
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human tropomyosin-2 (NP_003280.2). MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for tropomyosin-2 Antibody - Azide and BSA Free
Western Blot: tropomyosin-2 AntibodyAzide and BSA Free [NBP2-95197]
Western Blot: tropomyosin-2 Antibody [NBP2-95197] - Analysis of extracts of various cell lines, using tropomyosin-2 at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.Immunohistochemistry-Paraffin: tropomyosin-2 Antibody - Azide and BSA Free [NBP2-95197]
Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-95197] - Rat heart using TPM2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: tropomyosin-2 Antibody - Azide and BSA Free [NBP2-95197]
Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-95197] - Human stomach using TPM2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for tropomyosin-2 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:50-1:100
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: tropomyosin-2
Alternate Names
distal, type 1, tropomyosin 2 (beta)
Gene Symbol
TPM2
Additional tropomyosin-2 Products
Product Documents for tropomyosin-2 Antibody - Azide and BSA Free
Product Specific Notices for tropomyosin-2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov