Troponin I Type 1 (slow skeletal) Antibody (12F10)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-431

Novus Biologicals, part of Bio-Techne
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NB200-431-0.2mg
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Key Product Details

Species Reactivity

Human

Applications

Immunoaffinity Purification, Immunoassay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 12F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I Type 1 (slow skeletal) Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Human skTnI.

Specificity

Troponin I from human skeletal muscle. Cross-reaction of this with cardiac troponin I is less then 0.05%.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Troponin I Type 1 (slow skeletal) Antibody (12F10)

Application
Recommended Usage

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pair for sandwich immunoassay (coating - conjugate): NB200-431 (Coating) - NB200-432 (conjugate)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I Type 1 (slow skeletal)

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Entrez Gene IDs

7135 (Rat)

Gene Symbol

TNNI1

UniProt

P19237 (Human)

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Troponin I Type 1 (slow skeletal) Antibody (12F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Troponin I Type 1 (slow skeletal) Antibody (12F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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