Troponin I Type 1 (slow skeletal) Antibody (5K1U2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16855

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16855-100ul
NBP3-16855-20ul
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5K1U2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Troponin I Type 1 (slow skeletal) Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human Troponin I Type 1 (slow skeletal) (P19237). MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Troponin I Type 1 (slow skeletal) Antibody (5K1U2)

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (5K1U2) [NBP3-16855]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (5K1U2) [NBP3-16855]

Western Blot: Troponin I Type 1 (slow skeletal) Antibody (5K1U2) [NBP3-16855] - Western blot analysis of extracts of Rat skeletal muscle cells, using Troponin I Type 1 (slow skeletal) antibody (NBP3-16855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for Troponin I Type 1 (slow skeletal) Antibody (5K1U2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I Type 1 (slow skeletal)

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Alternate Names

DKFZp451O223, slow-twitch isoform, SSTNI, TNN1, troponin I type 1 (skeletal, slow), troponin I, skeletal, slow, troponin I, slow skeletal muscle

Gene Symbol

TNNI1

Additional Troponin I Type 1 (slow skeletal) Products

Product Documents for Troponin I Type 1 (slow skeletal) Antibody (5K1U2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin I Type 1 (slow skeletal) Antibody (5K1U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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