Troponin I type 2 (fast skeletal) Antibody (2D5)

Novus Biologicals, part of Bio-Techne | Catalog # H00007136-M05

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
H00007136-M05-50ug
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I type 2 (fast skeletal) Antibodies »

Product Specifications

Immunogen

TNNI2 (NP_003273.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GDEEKRNRAITARRQHLKSVMLQIAATELEKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEEKYDMEVRVQKTSKELEDMNQKL

Specificity

Reacts with troponin I type 2 (skeletal, fast).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody (2D5)

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05] - Analysis of TNNI2 expression in transfected 293T cell line by TNNI2 monoclonal antibody (M05), clone 2D5. Lane 1: TNNI2 transfected lysatE (21.3 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Troponin I type 2 (fast skeletal) Antibody (2D5) [H00007136-M05] - Detection limit for recombinant GST tagged TNNI2 is 3 ng/ml as a capture antibody.

Applications for Troponin I type 2 (fast skeletal) Antibody (2D5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Troponin I type 2 (fast skeletal)

This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Alternate Names

skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform

Entrez Gene IDs

7136 (Rat)

Gene Symbol

TNNI2

UniProt

NP_003273 (Human)

Additional Troponin I type 2 (fast skeletal) Products

Product Documents for Troponin I type 2 (fast skeletal) Antibody (2D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody (2D5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.