Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3A11

Concentration

1 mg/ml

View all Troponin I type 2 (fast skeletal) Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TNNI2 (NP_003273) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

Western Blot: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Troponin I type 2.
Immunohistochemistry: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Immunohistochemistry: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Immunohistochemistry: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (OTI3A11) [NBP2-46171]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (3A11) [NBP2-46171] - Analysis of extracts (10ug) from HepG2 cell line.

Applications for Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin I type 2 (fast skeletal)

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Alternate Names

skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform

Gene Symbol

TNNI2

Additional Troponin I type 2 (fast skeletal) Products

Product Documents for Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody (OTI3A11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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