Troponin I Type 3 (cardiac) Antibody (1E7)
Novus Biologicals, part of Bio-Techne | Catalog # H00007137-M04
![Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-1E7-Western-Blot-H00007137-M04-img0001.jpg "Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]")
![ELISA: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-1E7-ELISA-H00007137-M04-img0008.jpg "ELISA: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]")
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TNNI3 (NP_000354, 102 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES
Specificity
TNNI3 - troponin I type 3 (cardiac)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (1E7)
![Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-1E7-Western-Blot-H00007137-M04-img0007.jpg "Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]")
Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]
Western Blot: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of TNNI3 expression in transfected 293T cell line by TNNI3 monoclonal antibody (M04), clone 1E7. Lane 1: TNNI3 transfected lysatE (24 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-1E7-Immunocytochemistry-Immunofluorescence-H00007137-M04-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]")
Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]
Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on HeLa cell. Antibody concentration 10 ug/ml![Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-Antibody-1E7-Immunohistochemistry-Paraffin-H00007137-M04-img0005.jpg "Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]")
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04]
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (1E7) [H00007137-M04] - Analysis of monoclonal antibody to TNNI3 on formalin-fixed paraffin-embedded human heart. Antibody concentration 0.7 ug/mlApplications for Troponin I Type 3 (cardiac) Antibody (1E7)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi validaton, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Troponin I/TNNI3
Long Name
Troponin I3, Cardiac Type
Alternate Names
CMD2A, CTnI, RCM1, TNNC1, TNNI3,
Entrez Gene IDs
7137 (Human)
Gene Symbol
TNNI3
OMIM
115210 (Human)
UniProt
Additional Troponin I/TNNI3 Products
Product Documents for Troponin I Type 3 (cardiac) Antibody (1E7)
Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (1E7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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