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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine (Negative), Canine (Negative), Feline (Negative), Fish (Negative), Goat (Negative), Mouse (Negative), Rabbit (Negative), Rat (Negative)

Applications

ELISA, Western Blot

Label

Janelia Fluor 585

Antibody Source

Monoclonal Mouse IgG2B Clone # 22B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Troponin I Type 3 (cardiac) Antibody (22B11) [Janelia Fluor® 585]

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cTnI.

Epitope

a.a.r. 20-24

Reactivity Notes

Cross-reacts with Human and Pig. Does not cross-react with Mouse, Rat, Cow, Cat, Dog, Fish, Goat or Rabbit. Not yet tested in other species.

Specificity

This detects the unphosphorylated form of cardiac troponin I.No cross-reaction with mono or biphosphorylated troponin I.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Troponin I Type 3 (cardiac) Antibody (22B11) [Janelia Fluor® 585]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Troponin I/TNNI3

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Gene Symbol

TNNI3

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (22B11) [Janelia Fluor® 585]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (22B11) [Janelia Fluor® 585]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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