Troponin I Type 3 (cardiac) Antibody (MF4cc)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-10239

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NB120-10239AF350

Alexa Fluor 532

NB120-10239AF532

Alexa Fluor 594

NB120-10239AF594

Alexa Fluor 750

NB120-10239AF750

Biotin

NB120-10239B

DyLight 594

NB120-10239DL594

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine, Canine, Feline, Fish (Negative), Goat, Rabbit (Negative)

Applications

ELISA, Immunoaffinity Purification, Immunoassay, Immunohistochemistry, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # MF4cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Troponin I/TNNI3 Antibodies »

Product Summary for Troponin I Type 3 (cardiac) Antibody (MF4cc)

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnI.

Epitope

a.a.r. 190-196

Reactivity Notes

Cross-reacts with Rat, Cow, Cat, Dog, Goat, Pig and Human. Does not cross-react with Fish or Rabbit. Not yet tested in other species.

Localization

Cytoplasmic

Specificity

No cross-reaction with skeletal TnT

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Troponin I Type 3 (cardiac) Antibody (MF4cc)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human); 29248 (Rat)

Gene Symbol

TNNI3

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (MF4cc)

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (MF4cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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