Troponin I Type 3 (cardiac) [p Ser43] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29515
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%), Human (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Troponin I Type 3 (cardiac) [p Ser43] Antibody
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser43 conjugated to KLH. Accession # P48787
Modification
p Ser43
Specificity
Specific for endogenous levels of the ~25 kDa Troponin I Type 3 (cardiac) protein phosphorylated at Ser43. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Troponin I Type 3 (cardiac) [p Ser43] Antibody
![Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515]](https://resources.bio-techne.com/images/products/Troponin-I-Type-3-cardiac-p-Ser43-Antibody-Western-Blot-NBP2-29515-img0001.jpg "Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515]")
Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515]
Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515] - Western blot of mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser43 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.Applications for Troponin I Type 3 (cardiac) [p Ser43] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Troponin I/TNNI3
Long Name
Troponin I3, Cardiac Type
Alternate Names
CMD2A, CTnI, RCM1, TNNC1, TNNI3,
Entrez Gene IDs
21954 (Mouse)
Gene Symbol
TNNI3
UniProt
Additional Troponin I/TNNI3 Products
Product Documents for Troponin I Type 3 (cardiac) [p Ser43] Antibody
Product Specific Notices for Troponin I Type 3 (cardiac) [p Ser43] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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