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Troponin T Type 2 (cardiac) Antibody (CL13403)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24557

Novus Biologicals, part of Bio-Techne
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NBP3-24557-100ul
NBP3-24557-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13403

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Troponin T Type 2 (cardiac) Antibody (CL13403)

Immunogen

This antibody was designed to target the recombinant protein Troponin T Type 2 (cardiac), with the precise immunogen sequence being withheld for proprietary reasons.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Troponin T Type 2 (cardiac) Antibody (CL13403)

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (CL13403) [NBP3-24557]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (CL13403) [NBP3-24557]

Staining of human small intestine shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Troponin T Type 2 (cardiac) Antibody (CL13403) [NBP3-24557]

Analysis in human heart muscle and skeletal muscle tissues using NBP3-24557 antibody. Corresponding TNNT2 RNA-seq data are presented for the same tissues.

Applications for Troponin T Type 2 (cardiac) Antibody (CL13403)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin T Type 2 (cardiac)

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Alternate Names

cardiac, CMD1D, CMPD2, dilated 1D (autosomal dominant), hypertrophic 2, MGC3889, RCM3, TnTc, troponin T type 2 (cardiac), troponin T, cardiac muscle

Gene Symbol

TNNT2

Additional Troponin T Type 2 (cardiac) Products

Product Documents for Troponin T Type 2 (cardiac) Antibody (CL13403)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Troponin T Type 2 (cardiac) Antibody (CL13403)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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