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Troponin T Type 3 (fast skeletal) Antibody (1F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00007140-M02

Novus Biologicals, part of Bio-Techne
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H00007140-M02

Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

TNNT3 (NP_006748, 161 a.a. ~ 258 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADQKRGKKQTAREMKKKILAERRKPLNIDHLGEDKLRDKAKELWETLHQLEIDKFEFGEKLKRQKYDITTLRSRIDQAQKHSKKAGTPAKGKVGGRWK

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 22444818).

Specificity

TNNT3 (1F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Troponin T Type 3 (fast skeletal) Antibody (1F12)

Sandwich ELISA: Troponin T Type 3 (fast skeletal) Antibody (1F12) [H00007140-M02] - Detection limit for recombinant GST tagged TNNT3 is approximately 0.1ng/ml as a capture antibody.

Applications for Troponin T Type 3 (fast skeletal) Antibody (1F12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Troponin T Type 3 (fast skeletal)

Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.

Alternate Names

Beta-TnTF, fast skeletal muscle, TnTf, troponin T type 3 (skeletal, fast), troponin T3, skeletal, fast, troponin-T3, skeletal, fast

Entrez Gene IDs

7140 (Rat)

Gene Symbol

TNNT3

OMIM

600692 (Human)

Additional Troponin T Type 3 (fast skeletal) Products

Product Documents for Troponin T Type 3 (fast skeletal) Antibody (1F12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Troponin T Type 3 (fast skeletal) Antibody (1F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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