Troponin T Type 3 (fast skeletal) Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26199

Novus Biologicals, part of Bio-Techne
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NBP2-26199
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Troponin T Type 3 (fast skeletal) Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TTLRSRIDQAQKHSK corresponding to C-Terminus according to NP_001036246.1, NP_001036247.1.

Specificity

This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Troponin T Type 3 (fast skeletal) Antibody

Western Blot: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199]

Western Blot: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199]

Western Blot: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199]

Immunohistochemistry-Paraffin: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199]

Immunohistochemistry-Paraffin: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Troponin T Type 3 (fast skeletal) Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 8 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in human, mouse and rat skeletal muscle lysates is reported in scientific literature (PMID: 9201030.). IHC-P: Human skeletal muscle shows staining of select fibrils.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Troponin T Type 3 (fast skeletal)

Cardiac Troponin T, a 36kDa protein, is the tropomyosin binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations within the protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the gene undergo alternative splicing that results in many tissue specific isoforms.

Alternate Names

Beta-TnTF, fast skeletal muscle, TnTf, troponin T type 3 (skeletal, fast), troponin T3, skeletal, fast, troponin-T3, skeletal, fast

Entrez Gene IDs

21957 (Mouse); 7140 (Rat)

Gene Symbol

TNNT3

Additional Troponin T Type 3 (fast skeletal) Products

Product Documents for Troponin T Type 3 (fast skeletal) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Troponin T Type 3 (fast skeletal) Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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