TRPC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20989

Novus Biologicals, part of Bio-Techne
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NBP1-20989
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TRPC4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KHAKEEDSSIDYD corresponding to internal region (near C-Terminus) according to NP_003297.1, NP_001129427.1, NP_001129429.1, NP_001129428.1, NP_001129430.1.

Epitope

C terminus

Specificity

This antibody is expected to recognise all six reported isoforms (NP_057263.1; NP_003297.1; NP_001129427.1; NP_001129429.1; NP_001129428.1; NP_001129430.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRPC4 Antibody

Western Blot: TRPC4 Antibody [NBP1-20989]

Western Blot: TRPC4 Antibody [NBP1-20989]

Western Blot: TRPC4 Antibody [NBP1-20989] - Staining of Rat Brain lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989]

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989]

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Placenta with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989]

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989]

Immunohistochemistry-Paraffin: TRPC4 Antibody [NBP1-20989] - Staining of paraffin embedded Human Kidney with antibody at 4.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TRPC4 Antibody

Application
Recommended Usage

Immunohistochemistry

4.75 ug/mL

Immunohistochemistry-Paraffin

4.75 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPC4

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective . May also be activated by intracellular calcium store depletion

Long Name

Short transient receptor potential channel 4

Alternate Names

hTrp-4, hTrp4

Entrez Gene IDs

7223 (Human); 22066 (Mouse); 84494 (Rat)

Gene Symbol

TRPC4

UniProt

NP_057263.1 (Human)

Additional TRPC4 Products

Product Documents for TRPC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRPC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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