Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # N67/15

Concentration

1 mg/ml

View all TRPC5 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)

Localization

Membrane

Specificity

Detects approx 110kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TRPC5 Antibody (N67/15)

Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919]

Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919]

Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for Overnight at 4C. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa.
Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody (N67/15) [NBP2-12919]

Immunocytochemistry/ Immunofluorescence: TRPC5 Antibody (N67/15) [NBP2-12919]

Immunocytochemistry/Immunofluorescence: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.
Immunohistochemistry: TRPC5 Antibody (N67/15) [NBP2-12919]

Immunohistochemistry: TRPC5 Antibody (N67/15) [NBP2-12919]

Immunohistochemistry: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.
View 1 more images

Applications for TRPC5 Antibody (N67/15)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

0.1-1.0 ug/ml

Western Blot

1:1000
Application Notes
1 ug/ml of TrpC5 Antibody was sufficient for detection of TrpC5 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPC5

1 ug/mL was sufficient for detection of TrpC5 in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Short transient receptor potential channel 5

Alternate Names

hTRP-5, hTRP5, TRP-5, TRP5

Gene Symbol

TRPC5

UniProt

NP_036603.1 (Human)

Additional TRPC5 Products

Product Documents for TRPC5 Antibody (N67/15)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRPC5 Antibody (N67/15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.