Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI10H5

Concentration

1 mg/ml

View all TRPM4 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.

Specificity

This antibody is specific for Homo sapiens transient receptor potential cation channel, subfamily M, member 4 (TRPM4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

134.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPM4 Antibody (OTI10H5)

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.
Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Immunohistochemistry-Paraffin: TRPM4 Antibody (OTI10H5) [NBP1-48036] - Human endometrial carcinoma tissue section. Fixed paraffin embedded sections were deparaffinized. EDTA was used for antigen retrieval. Samples were incubated O/N in 4 C with the primary antibody (1:200 dilution). IHC-P image submitted by a verified review.
Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (OTI10H5) [NBP1-48036]

Western Blot: TRPM4 Antibody (10H5) [NBP1-48036] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4.
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Applications for TRPM4 Antibody (OTI10H5)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000-10000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-48036 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPM4

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload

Long Name

Transient receptor potential cation channel subfamily M member 4

Alternate Names

hTRPM4, LTrpC-4, LTrpC4, Melastatin-4

Entrez Gene IDs

54795 (Human)

Gene Symbol

TRPM4

Additional TRPM4 Products

Product Documents for TRPM4 Antibody (OTI10H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRPM4 Antibody (OTI10H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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