Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S74

Concentration

1 mg/ml

View all TRPM7 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7

Localization

Membrane

Specificity

Detects approx 220kDa. No cross-reactivity against TrpM6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TRPM7 Antibody (S74)

Western Blot: TRPM7 Antibody (S74) [NBP2-12906]

Western Blot: TRPM7 Antibody (S74) [NBP2-12906]

Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906]

Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906]

Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.
Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906]

Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906]

Immunohistochemistry: TRPM7 Antibody (S74) [NBP2-12906] - Immunohistochemistry analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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Applications for TRPM7 Antibody (S74)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of TrpM7 Antibody was sufficient for detection of TrpM7 in 10 ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPM7

1 ug/mL was sufficient for detection of TrpM7 in 10ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Alternate Names

CHAK, CHAK1FLJ25718, Channel-kinase 1, EC 2.7.11.1, Long transient receptor potential channel 7, LTRPC ion channel family member 7, LTrpC7, LTrpC-7, LTRPC7FLJ20117, transient receptor potential cation channel subfamily M member 7, transient receptor potential cation channel, subfamily M, member 7, transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK

Gene Symbol

TRPM7

Additional TRPM7 Products

Product Documents for TRPM7 Antibody (S74)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRPM7 Antibody (S74)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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