TRPM7 [p Ser1513] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23186
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-TRPM7 (Ser-1513) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members. [UniProt# Q96QT4]
Modification
p Ser1513
Specificity
The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1513) peptide then affinity purified using phospho-TRPM7 (Ser-1513) peptide. This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 and rat PC12 cells stimulated with calyculin A, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for TRPM7 [p Ser1513] Antibody
![TRPM7 [p Ser1513] Antibody](https://resources.bio-techne.com/images/products/nbp3-23186_rabbit-trpm7-p-ser1513-pab-199202312523634.jpg "Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] -")
Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] -
Western Blot: TRPM7 [p Ser1513] Antibody [NBP3-23186] - Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) NBP3-23362 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).Applications for TRPM7 [p Ser1513] Antibody
Application
Recommended Usage
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: TRPM7
Alternate Names
CHAK, CHAK1FLJ25718, Channel-kinase 1, EC 2.7.11.1, Long transient receptor potential channel 7, LTRPC ion channel family member 7, LTrpC7, LTrpC-7, LTRPC7FLJ20117, transient receptor potential cation channel subfamily M member 7, transient receptor potential cation channel, subfamily M, member 7, transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK
Gene Symbol
TRPM7
Additional TRPM7 Products
Product Documents for TRPM7 [p Ser1513] Antibody
Product Specific Notices for TRPM7 [p Ser1513] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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