TRUB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80866

Novus Biologicals, part of Bio-Techne
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NBP1-80866
NBP1-80866-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Hamster, Primate - Macaca mulatta (Rhesus Macaque)

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRUB1 Antibodies »

Product Summary for TRUB1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRUB1 Antibody

Western Blot: TRUB1 Antibody [NBP1-80866]

Western Blot: TRUB1 Antibody [NBP1-80866]

Western Blot: TRUB1 Antibody [NBP1-80866] - Analysis in control (vector only transfected HEK293T lysate) and TRUB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TRUB1 Antibody [NBP1-80866]

Immunocytochemistry/ Immunofluorescence: TRUB1 Antibody [NBP1-80866]

Immunocytochemistry/Immunofluorescence: TRUB1 Antibody [NBP1-80866] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRUB1 Antibody [NBP1-80866]

Immunohistochemistry-Paraffin: TRUB1 Antibody [NBP1-80866]

Immunohistochemistry-Paraffin: TRUB1 Antibody [NBP1-80866] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
View 3 more images

Applications for TRUB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRUB1

Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).[supplied by OMIM]

Alternate Names

EC 5.4.99, EC 5.4.99.-, PUS4probable tRNA pseudouridine synthase 1, TruB pseudouridine (psi) synthase homolog 1 (E. coli)

Entrez Gene IDs

142940 (Human)

Gene Symbol

TRUB1

Additional TRUB1 Products

Product Documents for TRUB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRUB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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