Trypsin 1/PRSS1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44111
Key Product Details
Species Reactivity
Validated:
Bovine
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Summary for Trypsin 1/PRSS1 Antibody
Immunogen
Trypsin 1/PRSS1 Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified. (Uniprot: P00760)
Reactivity Notes
Cross-reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but has not been specifically determined.
Specificity
Cross-reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but has not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin (Bovine Pancreas)
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Trypsin 1/PRSS1 Antibody
![Western Blot: Trypsin 1/PRSS1 Antibody [NBP2-44111]](https://resources.bio-techne.com/images/products/Trypsin-1-PRSS1-Antibody-Western-Blot-NBP2-44111-img0002.jpg "Western Blot: Trypsin 1/PRSS1 Antibody [NBP2-44111]")
Western Blot: Trypsin 1/PRSS1 Antibody [NBP2-44111]
Western Blot: Trypsin 1/PRSS1 Antibody [NBP2-44111] - Western Blot of Trypsin 1/PRSS1 1/PRSS1 antibody. Lane 1: Trypsin 1/PRSS1 (Bovine). Load: 50 ng per lane.Primary antibody: Trypsin 1/PRSS1 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 kDa for Trypsin 1/PRSS1. Other band(s): Low molecular weight bands could correspond to autocatalytic fragments.Applications for Trypsin 1/PRSS1 Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Western Blot
1:1000-1:5000
Application Notes
This product has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Trypsin 1/PRSS1
Alternate Names
PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1
Gene Symbol
PRSS1
UniProt
Additional Trypsin 1/PRSS1 Products
Product Documents for Trypsin 1/PRSS1 Antibody
Product Specific Notices for Trypsin 1/PRSS1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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