Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01679

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G1

Concentration

1 mg/ml

View all Tryptase gamma-1/TPSG1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 20-283 of human TPSG1(NP_036599) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

Western Blot: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) [NBP2-01679]

Western Blot: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) [NBP2-01679]

Western Blot: Tryptase gamma Antibody (1G1) [NBP2-01679] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tryptase gamma (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tryptase gamma.
Immunohistochemistry-Paraffin: Tryptase gamma Antibody (1G1) [NBP2-01679] Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Tryptase gamma mouse monoclonal antibody.
Flow Cytometry: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) [NBP2-01679]

Flow Cytometry: Tryptase gamma-1/TPSG1 Antibody (OTI1G1) [NBP2-01679]

Flow Cytometry: Tryptase gamma Antibody (1G1) [NBP2-01679] - Analysis of Jurkat cells, using anti-Tryptase gamma antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tryptase gamma-1/TPSG1

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Alternate Names

TPSG1, Tryptase gamma1

Entrez Gene IDs

25823 (Human)

Gene Symbol

TPSG1

Additional Tryptase gamma-1/TPSG1 Products

Product Documents for Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptase gamma-1/TPSG1 Antibody (OTI1G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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