Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90022
Recombinant Monoclonal Antibody
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]](https://resources.bio-techne.com/images/products/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 145
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1/TPH-1 (Uniprot#: P17752-1; Ile2-Ile444).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]
Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]](https://resources.bio-techne.com/images/products/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg "Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]")
Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022]
Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDaApplications for Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
Application
Recommended Usage
Immunoprecipitation
0.2-1 ul/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tryptophan Hydroxylase 1/TPH-1
Alternate Names
TPH-1, TPRH
Gene Symbol
TPH1
Additional Tryptophan Hydroxylase 1/TPH-1 Products
Product Documents for Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 Antibody (145)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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