Tryptophan Hydroxylase 1/TPH-1 Antibody (TPH1/7661) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27018

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 kappa Clone # TPH1/7661

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Tryptophan Hydroxylase 1/TPH-1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Tryptophan Hydroxylase 1/TPH-1 protein

Localization

Cytoplasm, Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2 kappa

Description

Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images

Tryptophan Hydroxylase 1/TPH-1 Antibody (TPH1/7661)

Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (TPH1/7661) - Azide and BSA Free [NBP3-27018] -

Formalin-fixed, paraffin-embedded human kidney cancer stained with Tryptophan Hydroxylase 1/TPH-1 Antibody (TPH1/7661) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. (1). TPH1 is phosphorylated by cAMP-dependent protein kinase A (2). Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy (3).

Alternate Names

TPH-1, TPRH

Gene Symbol

TPH1

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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