Tryptophan Hydroxylase 1/TPH-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-176

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB300-176
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit (Negative)

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Equine (100%), Feline (100%), Goat (100%), Guinea Pig (100%), Hamster (100%), Primate (100%), Sheep (100%), Vole (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tryptophan Hydroxylase 1/TPH-1 Antibodies »

Product Specifications

Immunogen

Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. Accession # P17290

Reactivity Notes

Reactivity assumed based on sequence identity to a wide variety of mammalian species. Mouse reactivity reported in scientific literature (PMID: 28456683).

Specificity

Specific for endogenous levels of the ~55 kDa Tryptophan Hydroxylase 1/TPH-1 protein.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 Antibody

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

Applications for Tryptophan Hydroxylase 1/TPH-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) is one of a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. It contains catalytic and regulatory domains, as well as a short tetramerization domain. TPH is the first catalytic step in the biosynthesis of serotonin in the CNS and melatonin in the pineal gland. TPH plays an important role in the pathology of several neuropsychiatric disorders, including depression, aggression, and schizophrenia.

Alternate Names

TPH-1, TPRH

Entrez Gene IDs

7166 (Human); 24848 (Rat)

Gene Symbol

TPH1

UniProt

P17752

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Tryptophan Hydroxylase 1/TPH-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.