Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-307

Novus Biologicals, part of Bio-Techne
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NB300-307
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Mouse (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Tryptophan Hydroxylase 1/TPH-1 Antibodies »

Product Summary for Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH. Accession # P09810

Modification

p Ser260

Localization

Cytoplasmic

Specificity

Specific for the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser260.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody [NB300-307]

Western Blot: Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody [NB300-307]

Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.

Applications for Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot in human dorsal Raphe nucleus, detecting a band ~55 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH.

Alternate Names

TPH-1, TPRH

Entrez Gene IDs

7166 (Human); 21990 (Mouse); 24848 (Rat)

Gene Symbol

TPH1

UniProt

P09810 (Rat)

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptophan Hydroxylase 1/TPH-1 [p Ser260] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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