TSC2 Antibody (S06-0D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14981

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14981-100ul
NBP3-14981-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S06-0D3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TSC2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human TSC2 (Uniprot#: P49815)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

201 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSC2 Antibody (S06-0D3)

Western Blot: TSC2 Antibody (S06-0D3) [NBP3-14981]

Western Blot: TSC2 Antibody (S06-0D3) [NBP3-14981]

Western Blot: TSC2 Antibody (S06-0D3) [NBP3-14981] - Western blot detection of TSC2 in Hela, A549, MCF-7, 293T cell lysates using TSC2 Rabbit mAb (1:1000 diluted). Predicted band size: 201kDa. Observed band size: 201kDa.
Immunohistochemistry: TSC2 Antibody (S06-0D3) [NBP3-14981]

Immunohistochemistry: TSC2 Antibody (S06-0D3) [NBP3-14981]

Immunohistochemistry: TSC2 Antibody (S06-0D3) [NBP3-14981] - Immunohistochemical of TSC2 in Human hepatic carcinoma tissue using TSC2 antibody at dilution 1/50

Applications for TSC2 Antibody (S06-0D3)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex-2 (TCS2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

Additional TSC2 Products

Product Documents for TSC2 Antibody (S06-0D3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSC2 Antibody (S06-0D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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