Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TSC2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Tuberous Sclerosis 2 using the numbering given in Swiss-Prot entry P49815 (GeneID 7249).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TSC2 Antibody

Immunoprecipitation: TSC2 Antibody [NB200-200]

Immunoprecipitation: TSC2 Antibody [NB200-200]

Immunoprecipitation: TSC2 Antibody [NB200-200] - Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody NB200-200 used for IP at 3 ug per reaction. TSC2 was also immunoprecipitated by another rabbit anti-TSC2 antibody. For blotting immunoprecipitated TSC2, NB200-200 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TSC2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TSC2

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Entrez Gene IDs

7249 (Human)

Gene Symbol

TSC2

UniProt

P49815

Additional TSC2 Products

Product Documents for TSC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TSC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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