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TSH beta Antibody (1D12G1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52574

Novus Biologicals, part of Bio-Techne
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NBP2-52574
NBP2-52574-0.025mg

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1D12G1

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human TSH beta (AA: 20-139) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSH beta Antibody (1D12G1) - BSA Free

Western Blot: TSH beta Antibody (1D12G1)BSA Free [NBP2-52574]

Western Blot: TSH beta Antibody (1D12G1)BSA Free [NBP2-52574]

Western Blot: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis using TSHB mAb against human TSHB (AA: 20-139) recombinant protein. (Expected MW is 16 kDa)
Immunohistochemistry-Paraffin: TSH beta Antibody (1D12G1) - BSA Free [NBP2-52574]

Immunohistochemistry-Paraffin: TSH beta Antibody (1D12G1) - BSA Free [NBP2-52574]

Immunohistochemistry-Paraffin: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis of paraffin-embedded pancreas tissues using TSHB mouse mAb with DAB staining.
Flow Cytometry: TSH beta Antibody (1D12G1) - BSA Free [NBP2-52574]

Flow Cytometry: TSH beta Antibody (1D12G1) - BSA Free [NBP2-52574]

Flow Cytometry: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis of Hela cells using TSHB mouse mAb (green) and negative control (red).

Applications for TSH beta Antibody (1D12G1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSH beta

Thyroid-stimulating hormone (TSH), also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non-covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle-stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Long Name

Thyroid-stimulating Hormone Subunit beta

Alternate Names

Thyrotropin beta, TSHB

Gene Symbol

TSHB

Additional TSH beta Products

Product Documents for TSH beta Antibody (1D12G1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TSH beta Antibody (1D12G1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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