TSLP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55233

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TSLP Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TSLP. The immunogen is located within the last 50 amino acids of TSLP. Amino Acid Sequence: ATQAMKKRRKRKVTTNK

Specificity

Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and rat TSLP also has one isoform (135aa, 16kD). NB110-55233 can detect only human isoforms, but cannot detect mouse and rat TSLP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSLP Antibody - BSA Free

Western Blot: TSLP AntibodyBSA Free [NB110-55233]

Western Blot: TSLP AntibodyBSA Free [NB110-55233]

Western Blot: TSLP Antibody [NB110-55233] - Analysis of TSLP in Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/mL
Western Blot: TSLP AntibodyBSA Free [NB110-55233]

Western Blot: TSLP AntibodyBSA Free [NB110-55233]

Western Blot: TSLP Antibody [NB110-55233] - Jurkat cell lysate with TSLP antibody at (A) 1 and (B) 2 ug/ml.
TSLP Antibody - BSA Free

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55233] -

Immunohistochemistry: TSLP Antibody - BSA Free [NB110-55233] - Figure 3 Immunohistochemistry Validation of TSLP in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-TSLP antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for TSLP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.25-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSLP

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human)

Gene Symbol

TSLP

UniProt

Q969D9

Additional TSLP Products

Product Documents for TSLP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSLP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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