TSLPR/CRLF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22384

Novus Biologicals, part of Bio-Techne
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NBP2-22384
NBP2-22384SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG2b Kappa

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TSLPR Antibodies »

Product Summary for TSLPR/CRLF2 Antibody

Immunogen

Amino acids 119-231 of human TSLPR protein were used as the immunogen for the antibody.

Clonality

Polyclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for TSLPR/CRLF2 Antibody

Western Blot: TSLPR/CRLF2 Antibody [NBP2-22384]

Western Blot: TSLPR/CRLF2 Antibody [NBP2-22384]

Western Blot: TSLP R/CRLF2 Antibody [NBP2-22384] - Analysis of TSLPR in an A) partial recombinant protein (0.1 ug/ml) and B) human liver lysate (2ug/ml) using TSLPR antibody.
Immunohistochemistry-Paraffin: TSLPR/CRLF2 Antibody [NBP2-22384]

Immunohistochemistry-Paraffin: TSLPR/CRLF2 Antibody [NBP2-22384]

Immunohistochemistry-Paraffin: TSLP R/CRLF2 Antibody [NBP2-22384] - Paraffin-embedded normal human kidney tissue stained with TSLPR antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

Applications for TSLPR/CRLF2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5-7 ug/ml

Western Blot

1-5 ug/ml
Application Notes
TSLPR is a glycoprotein with two major isoforms of 359 amino acids and 255 amino acids. In western blotting, bands may be visualized between 27 kD and ~50 kD.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSLPR

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgammac.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

Long Name

Thymic Stromal Lymphopoietin Receptor

Alternate Names

CRL2, CRLF2, CRLM-2, PCOR1, TSLP R

Gene Symbol

CRLF2

UniProt

AF338733_1 (Human)

Additional TSLPR Products

Product Documents for TSLPR/CRLF2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSLPR/CRLF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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