TST Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98573

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TST Antibodies »

Product Summary for TST Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human TST fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TST Antibody

Western Blot: TST Antibody [NBP2-98573]

Western Blot: TST Antibody [NBP2-98573]

Western Blot: TST Antibody [NBP2-98573] - Anti-TST rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 33 kDa
Immunocytochemistry/ Immunofluorescence: TST Antibody [NBP2-98573]

Immunocytochemistry/ Immunofluorescence: TST Antibody [NBP2-98573]

Immunocytochemistry/Immunofluorescence: TST Antibody [NBP2-98573] - Immunofluorescence staining of TST in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TST polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: TST Antibody [NBP2-98573]

Immunohistochemistry-Paraffin: TST Antibody [NBP2-98573]

Immunohistochemistry-Paraffin: TST Antibody [NBP2-98573] - Immunochemical staining of human TST in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
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Applications for TST Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TST

The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. The gene product contains two highly conservative domains (rhodanese homology domains), suggesting these domains have a common evolutionary origin.

Alternate Names

EC 2.8.1, EC 2.8.1.1, MGC19578, RDSRhodanese, thiosulfate sulfurtransferase, thiosulfate sulfurtransferase (rhodanese)

Gene Symbol

TST

Additional TST Products

Product Documents for TST Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TST Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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