TSTA3 Antibody (2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00007264-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TSTA3 Antibodies »

Product Specifications

Immunogen

TSTA3 (NP_003304, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DLAQLFIWVLREYNEVEPIILSVGEEDEVSIKEAAEAVVEAMDFHGEVTFDTTKSDGQFKKTASNSKLRTYLPDFRFTPFKQAVKETCAWFTDNYEQARK

Specificity

TSTA3 (2B9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TSTA3 Antibody (2B9)

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01] - TSTA3 monoclonal antibody (M01), clone 2B9 Analysis of TSTA3 expression in HeLa.
Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01] - Analysis of TSTA3 over-expressed 293 cell line, cotransfected with TSTA3 Validated Chimera RNAi ( Cat # H00007264-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TSTA3 monoclonal antibody (M01), clone 2B9 (Cat # H00007264-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01]

Western Blot: TSTA3 Antibody (2B9) [H00007264-M01] - Analysis of TSTA3 expression in transfected 293T cell line by TSTA3 monoclonal antibody (M01), clone 2B9.Lane 1: TSTA3 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.
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Applications for TSTA3 Antibody (2B9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TSTA3

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.

Alternate Names

EC 1.1.1.271, FX, GDP-4-keto-6-deoxy-D-mannose epimerase-reductase, GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, GDP-L-fucose synthase, P35B, Protein FX, Red cell NADP(H)-binding protein, SDR4E13-5 epimerase/4-reductase, short chain dehydrogenase/reductase family 4E, member 1, Short-chain dehydrogenase/reductase family 4E member 1, tissue specific transplantation antigen 3, tissue specific transplantation antigen P35B, Tissue-specific transplantation antigen-3

Entrez Gene IDs

7264 (Human)

Gene Symbol

TSTA3

OMIM

137020 (Human)

UniProt

NP_003304 (Human)

Additional TSTA3 Products

Product Documents for TSTA3 Antibody (2B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TSTA3 Antibody (2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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