TTC19 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98556
![Western Blot: TTC19 Antibody [NBP2-98556]](https://resources.bio-techne.com/images/products/TTC19-Antibody-Western-Blot-NBP2-98556-img0003.jpg "Western Blot: TTC19 Antibody [NBP2-98556]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human TTC19 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TTC19 Antibody
Western Blot: TTC19 Antibody [NBP2-98556]
Western Blot: TTC19 Antibody [NBP2-98556] - Lane A: HT-1080 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:34 kDa![Immunocytochemistry/ Immunofluorescence: TTC19 Antibody [NBP2-98556]](https://resources.bio-techne.com/images/products/TTC19-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98556-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TTC19 Antibody [NBP2-98556]")
Immunocytochemistry/ Immunofluorescence: TTC19 Antibody [NBP2-98556]
Immunocytochemistry/Immunofluorescence: TTC19 Antibody [NBP2-98556] - Immunofluorescence staining of TTC19 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TTC19 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.![Immunoprecipitation: TTC19 Antibody [NBP2-98556]](https://resources.bio-techne.com/images/products/TTC19-Antibody-Immunoprecipitation-NBP2-98556-img0004.jpg "Immunoprecipitation: TTC19 Antibody [NBP2-98556]")
Immunoprecipitation: TTC19 Antibody [NBP2-98556]
Immunoprecipitation: TTC19 Antibody [NBP2-98556] - Lane A:0.5 mg U-251MG Whole Cell Lysate4 uL anti-TTC19 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TTC19 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :34 kDaApplications for TTC19 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TTC19
Alternate Names
MGC138312, MGC19520, tetratricopeptide repeat domain 19,2010204O13Rik, tetratricopeptide repeat protein 19, mitochondrial, TPR repeat protein 19
Gene Symbol
TTC19
Additional TTC19 Products
Product Specific Notices for TTC19 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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