Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI10F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TTF-1/NKX2-1 Antibodies »

Product Summary for TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human NKX2-1(NP_003308) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free

Western Blot: TTF-1 / NKX2-1 Antibody (OTI10F8)Azide and BSA Free [NBP2-74667]

Western Blot: TTF-1 / NKX2-1 Antibody (OTI10F8)Azide and BSA Free [NBP2-74667]

Western Blot: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NKX2-1.
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667] - Staining of NCI-H1975 cells using anti-NKX2-1 mouse monoclonal antibody . The right is A549 cells as negative control.
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free [NBP2-74667] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (OTI10F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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