TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20691
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # TTF1/8427
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
Immunogen
Recombinant human TTF-1 / NKX2-1 protein
Localization
Nucleus.
Specificity
Anti-TTF-1 / NKX2-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Positive Controls: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
TTF-1 / NKX2-1 Antibody (TTF1/8427)
Formalin-fixed, paraffin-embedded human thyroid stained with TTF-1 / NKX2-1 antibody (TTF1/8427). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
TTF-1 / NKX2-1 Antibody (TTF1/8427)
Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with TTF-1 / NKX2-1 antibody (TTF1/8427). Inset: PBS instead of primary antibody; secondary only negative control.Applications for TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: TTF-1/NKX2-1
Long Name
NK2 Homeobox 1
Alternate Names
BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1
Gene Symbol
NKX2-1
Additional TTF-1/NKX2-1 Products
Product Documents for TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
Product Specific Notices for TTF-1 / NKX2-1 Antibody (TTF1/8427) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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