TTF-1 / NKX2-1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41160

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TTF-1/NKX2-1 Antibodies »

Product Summary for TTF-1 / NKX2-1 Antibody - BSA Free

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1. The immunogen is located within the first 50 amino acids of NKX2-1. Amino Acid Squence: LSPLEESYKKVGMEGG

Specificity

NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41, 45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TTF-1 / NKX2-1 Antibody - BSA Free

Western Blot: TTF-1 / NKX2-1 AntibodyBSA Free [NBP2-41160]

Western Blot: TTF-1 / NKX2-1 AntibodyBSA Free [NBP2-41160]

Western Blot: TTF-1 / NKX2-1 Antibody [NBP2-41160] - Analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 ug/ml.
Immunocytochemistry/ Immunofluorescence: TTF-1 / NKX2-1 Antibody - BSA Free [NBP2-41160]

Immunocytochemistry/ Immunofluorescence: TTF-1 / NKX2-1 Antibody - BSA Free [NBP2-41160]

Immunocytochemistry/Immunofluorescence: TTF-1 / NKX2-1 Antibody [NBP2-41160] - Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 ug/mL.
Immunohistochemistry: TTF-1 / NKX2-1 Antibody - BSA Free [NBP2-41160]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody - BSA Free [NBP2-41160]

Immunohistochemistry: TTF-1 / NKX2-1 Antibody [NBP2-41160] - Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 ug/mL.

Applications for TTF-1 / NKX2-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/mL

Western Blot

0.25 - 0.5 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TTF-1 / NKX2-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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