Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G5

Concentration

0.42 mg/ml

View all TTL Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TTL (NP_714923) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TTL Antibody (OTI3G5)

Western Blot: TTL Antibody (3G5) [NBP2-03288] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTL.

Applications for TTL Antibody (OTI3G5)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.42 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TTL

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM]. COMPLETENESS: complete on the 3' end.

Alternate Names

EC 6.3.2.25,2410003M22Rik, MGC46235, tubulin tyrosine ligase, tubulin-tyrosine ligase, tubulin--tyrosine ligase

Entrez Gene IDs

150465 (Human)

Gene Symbol

TTL

Additional TTL Products

Product Documents for TTL Antibody (OTI3G5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TTL Antibody (OTI3G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.